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mdcs (5 × 10 5 cells/ml)  (Jackson Laboratory)
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Jackson Laboratory mdcs (5 × 10 5 cells/ml)
β-glucan-stimulated <t>mDCs</t> can suppress both IL-5 and IFNγ production from Th2-primed Bet v 1-specific CD4 + T cells. Bone marrow <t>of</t> <t>BALB/c</t> mice was isolated and differentiated into mDCs for 8 days. CD4 + T cells were isolated from BALB/c mice that were previously sensitized twice with 10 µg of the major birch pollen allergen Bet v 1 and 2 mg Alum i.p. The differentiated mDCs and the isolated T cells were co-cultured in 48-well plates and either stimulated with 8 µg of the respective β-glucan (lighter colors) or re-stimulated with 4.3 µg Bet v 1 in the presence of 8 µg of the respective β-glucan (darker colors) for additional 72 h ( A ). The Warburg Effect was measured at OD 570nm normalized to the unstimulated controls with or without Bet v 1, respectively ( B ). Secretion of IL-2, IL-5, IFNγ, IL-10, and IL-13 were determined via sandwich ELISA. Data are mean results ± SD of three independent experiments. Statistical comparison was performed by 1-way ANOVA with correction for multiple comparisons according to Dunnett and indicated as follows: no indication = not significant and p -value > 0.05, * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, or **** p -value < 0.0001.
Mdcs (5 × 10 5 Cells/Ml), supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β-glucan-stimulated mDCs can suppress both IL-5 and IFNγ production from Th2-primed Bet v 1-specific CD4 + T cells. Bone marrow of BALB/c mice was isolated and differentiated into mDCs for 8 days. CD4 + T cells were isolated from BALB/c mice that were previously sensitized twice with 10 µg of the major birch pollen allergen Bet v 1 and 2 mg Alum i.p. The differentiated mDCs and the isolated T cells were co-cultured in 48-well plates and either stimulated with 8 µg of the respective β-glucan (lighter colors) or re-stimulated with 4.3 µg Bet v 1 in the presence of 8 µg of the respective β-glucan (darker colors) for additional 72 h ( A ). The Warburg Effect was measured at OD 570nm normalized to the unstimulated controls with or without Bet v 1, respectively ( B ). Secretion of IL-2, IL-5, IFNγ, IL-10, and IL-13 were determined via sandwich ELISA. Data are mean results ± SD of three independent experiments. Statistical comparison was performed by 1-way ANOVA with correction for multiple comparisons according to Dunnett and indicated as follows: no indication = not significant and p -value > 0.05, * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, or **** p -value < 0.0001.

Journal: International Journal of Molecular Sciences

Article Title: Characterization of the Immune-Modulating Properties of Different β-Glucans on Myeloid Dendritic Cells

doi: 10.3390/ijms25189914

Figure Lengend Snippet: β-glucan-stimulated mDCs can suppress both IL-5 and IFNγ production from Th2-primed Bet v 1-specific CD4 + T cells. Bone marrow of BALB/c mice was isolated and differentiated into mDCs for 8 days. CD4 + T cells were isolated from BALB/c mice that were previously sensitized twice with 10 µg of the major birch pollen allergen Bet v 1 and 2 mg Alum i.p. The differentiated mDCs and the isolated T cells were co-cultured in 48-well plates and either stimulated with 8 µg of the respective β-glucan (lighter colors) or re-stimulated with 4.3 µg Bet v 1 in the presence of 8 µg of the respective β-glucan (darker colors) for additional 72 h ( A ). The Warburg Effect was measured at OD 570nm normalized to the unstimulated controls with or without Bet v 1, respectively ( B ). Secretion of IL-2, IL-5, IFNγ, IL-10, and IL-13 were determined via sandwich ELISA. Data are mean results ± SD of three independent experiments. Statistical comparison was performed by 1-way ANOVA with correction for multiple comparisons according to Dunnett and indicated as follows: no indication = not significant and p -value > 0.05, * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, or **** p -value < 0.0001.

Article Snippet: BALB/c (Jackson Laboratories, Bar Harbor, ME, USA) mDCs (5 × 10 5 cells/mL) were cultured in 48-well plates with splenic CD4 + T cells (6.3 × 10 5 cells/mL) isolated from BALB/c mice sensitized with Bet v 1 plus aluminum hydroxide (Alum) (2 times 10 µg Bet v 1 plus 0.5 mg Alum i.p., 2 weeks apart, approval number: F107/1049, Regierungspräsidium Darmstadt) using the CD4 + T Cell Isolation Kit (Miltenyi Biotech, Bergisch Gladbach, Germany).

Techniques: Isolation, Cell Culture, Sandwich ELISA, Comparison